Insertion junction: CLIP2.007057_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.007057
Chromosome:chromosome 1
Location:2595705
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g015350 POR1,POR Light-dependent protochlorophyllide reductase; (1 of 1) K00218 - protochlorophyllide reductase (E1.3.1.33, por) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGCACTTCAATAATCTTATGCTGCTGTATGTAGCATGCGCGGGTAACAA
Internal bar code:GGGATGAGAATTCATTGCAGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1365
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:24
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:24

Suggested primers for confirmation by PCR

Suggested primer 1:GCGTCTTGTGGTGTTGGTTC
Suggested primer 2:GACATCTCTGCCAAGCTGGT