| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.007080 |
| Chromosome: | chromosome 8 |
| Location: | 3712604 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g380350 | (1 of 117) IPR002893 - Zinc finger, MYND-type | 3'UTR | |
| Cre08.g380400 | MAD2 | (1 of 1) K02537 - mitotic spindle assembly checkpoint protein MAD2 (MAD2); Mitotic spindle assembly checkpoint protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCTTGAGGAGTTGAGCGAGCAGGGTGAAGGATGTATGCACAAGGGCCA |
| Internal bar code: | GTGTGTCAAATCATGTACGACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2086 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCCTCATCTCCCCTCATTC |
| Suggested primer 2: | AACATCAAGAACGCGGAGGT |