| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.007102 |
| Chromosome: | chromosome 3 |
| Location: | 4042080 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g171900 | FAP56 | Flagellar Associated Protein 56 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAGGAGCGCGAGATTAACCAGATGCTGCAGCACGAGACGCAGCGCAAGG |
| Internal bar code: | TTAGTCAAGCTCGTTGGATGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1306 |
| LEAP-Seq percent confirming: | 72.7273 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTAGGTGCGTGCTGGGTAAG |
| Suggested primer 2: | TCCACCCCATTGCGTTCTAC |