| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.007119 |
| Chromosome: | chromosome 9 |
| Location: | 1471611 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g399914 | SSA8 | cilia-sensing, structure and/or assembly; (1 of 1) K10766 - alkylated DNA repair protein alkB homolog 4 (ALKBH4) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAGAGTGCAGCACTGGGGCCTGGAGCGACACGTCGATGAAACGACGCAC |
| Internal bar code: | CTTGGGAGAAGGAGTGAGGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3200 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 56 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 56 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATAAAGGTGAAGAGCGCG |
| Suggested primer 2: | AGTAGGGACTGAGAGGGCAG |