Insertion junction: CLIP2.007124_3

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.007124
Chromosome:chromosome 8
Location:580593
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g359900 (1 of 19) K08850 - aurora kinase, other [EC:2.7.11.1] (AURKX) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACAAGAAGCTCCGATGAACGGGCGCGCGCAGACGAGTGCACTAACCGTC
Internal bar code:AGTTGCGTCCGATTTAGTCTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:2828
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:28
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:28

Suggested primers for confirmation by PCR

Suggested primer 1:AAGCAGTGCAGCGAAACTTG
Suggested primer 2:TGCTCAGCTTGGACTTCAGG