| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.007124 |
| Chromosome: | chromosome 8 |
| Location: | 580641 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g359900 | (1 of 19) K08850 - aurora kinase, other [EC:2.7.11.1] (AURKX) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGAAGGTCGGGTTAACGGCACACCTGTCGCGCATCGGCCCTAACCCGG |
| Internal bar code: | AGTTGCGTCCGATTTAGTCTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2262 |
| LEAP-Seq percent confirming: | 66.6667 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTCAGCTTGGACTTCAGG |
| Suggested primer 2: | AAGCAGTGCAGCGAAACTTG |