| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.007132 |
| Chromosome: | chromosome 1 |
| Location: | 2763288 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g016750 | PSBS2 | (1 of 2) K03542 - photosystem II 22kDa protein (psbS); chloroplast Photosystem II-associated 22 kDa protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCCAACACAAGTATCCCACACACGCATACGCCAGTTGTCAAGCCAGCG |
| Internal bar code: | TTTACGGAATTCGTCGACCTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3066 |
| LEAP-Seq percent confirming: | 85.7143 |
| LEAP-Seq n confirming: | 48 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 56 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAGACCGATTTTGCGTGCG |
| Suggested primer 2: | AATCAAGGGGCGTGGCATAA |