Insertion junction: CLIP2.007140_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.007140
Chromosome:chromosome 13
Location:130250
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g562750 CGLD38 Conserved in the Green Lineage and Diatoms; (1 of 1) PF14234 - Domain of unknown function (DUF4336) (DUF4336) 5'UTR
Cre13.g562800 FAP337 (1 of 1) PTHR22844//PTHR22844:SF191 - F-BOX AND WD40 DOMAIN PROTEIN // SUBFAMILY NOT NAMED; WD40-repeat Flagellar Associated Protein 337 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGCATTAACAGGCGATAGCATGTTTAACAACTAGGTCGTGTGCAAGCTG
Internal bar code:ACACAGGGGCCTTCACCGTATT

Confirmation - LEAP-Seq

LEAP-Seq distance:1521
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:AGAATTGAGCGGTGGTCAGG
Suggested primer 2:CGGAAGAAAACCGTGTGCAG