| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.007140 |
| Chromosome: | chromosome 13 |
| Location: | 130252 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g562750 | CGLD38 | Conserved in the Green Lineage and Diatoms; (1 of 1) PF14234 - Domain of unknown function (DUF4336) (DUF4336) | 5'UTR |
| Cre13.g562800 | FAP337 | (1 of 1) PTHR22844//PTHR22844:SF191 - F-BOX AND WD40 DOMAIN PROTEIN // SUBFAMILY NOT NAMED; WD40-repeat Flagellar Associated Protein 337 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTGCGCCTATGCGTTCCGGGGGTCCTAGAAAGTCCTCTGTGCGCTGCAA |
| Internal bar code: | TTACATGCAACTTTTCGGGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 477 |
| LEAP-Seq percent confirming: | 36.8421 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGAAGAAAACCGTGTGCAG |
| Suggested primer 2: | AGAATTGAGCGGTGGTCAGG |