| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.007174 |
| Chromosome: | chromosome 7 |
| Location: | 3722365 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g338000 | MCM2 | (1 of 1) K02540 - DNA replication licensing factor MCM2 (MCM2); Minichromosome maintenance protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACGTGTCTATCGCCATCACCGCCTGCTGACCCGGCCCGCATACAGGAC |
| Internal bar code: | CTGTCCTATAGCGAATTTTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 805 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACGTGGCGTGAGTTATGGT |
| Suggested primer 2: | CACCCTGCAGGAGCTATACG |