Insertion junction: CLIP2.007179_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.007179
Chromosome:chromosome 12
Location:6938851
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g561500 3'UTR
Cre12.g561550 CDS1 Mitochondrial half-size ABC transporter, membrane protein; (1 of 2) K05661 - ATP-binding cassette, subfamily B (MDR/TAP), member 6 (ABCB6) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTCTTTATTCACTTGTGCCCCGGCGGGCGGACAGGATTCGCTCTCCAAC
Internal bar code:TAAGATCAGTTAAAGTTTGAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:2971
LEAP-Seq percent confirming:46.5116
LEAP-Seq n confirming:20
LEAP-Seq n nonconfirming:23
LEAP-Seq n unique pos:43

Suggested primers for confirmation by PCR

Suggested primer 1:CGAGAAGCTTCGCTGTCAGA
Suggested primer 2:CCTGGCTGGTAGTTTGCTGA