Insertion junction: CLIP2.007180_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.007180
Chromosome:chromosome 6
Location:7433107
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g300500 ADCY1,CYA18 Adenylyl cyclase; (1 of 1) PTHR11347//PTHR11347:SF132 - CYCLIC NUCLEOTIDE PHOSPHODIESTERASE // PDE1C, ISOFORM E CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCGATGGAAGCAACGGAATGTCGCCGACCACCCCAGATGTCGCCACCGG
Internal bar code:GAATTGAAGGTCTTATTATTAA

Confirmation - LEAP-Seq

LEAP-Seq distance:222
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GGTCTGAATGTTCATGCGGC
Suggested primer 2:GACCAAAGCACGCAACACAT