| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.007182 |
| Chromosome: | chromosome 3 |
| Location: | 5990560 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g189450 | (1 of 2) PTHR22769//PTHR22769:SF33 - MUTT/NUDIX HYDROLASE // NUCLEOSIDE DIPHOSPHATE-LINKED MOIETY X MOTIF 17 | 3'UTR | |
| Cre03.g189500 | (1 of 3) K03574 - 8-oxo-dGTP diphosphatase [EC:3.6.1.55] (mutT, NUDT15, MTH2) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAAAAGCTTCGCCCCTTCGCACTACAAGGTGAACTCCCCACCCACCCCCT |
| Internal bar code: | AGTTCAAAGACAAGCACGCTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2125 |
| LEAP-Seq percent confirming: | 80.0 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTGAACCCAGGCCAGTAT |
| Suggested primer 2: | TATGGCCGCATGATGCAGAT |