Insertion junction: CLIP2.007203_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.007203
Chromosome:chromosome 12
Location:4130918
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g517150 MET16,APSR1,APR1 Adenylylphosphosulfate reductase; (1 of 1) 1.8.99.2 - Adenylyl-sulfate reductase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTTGCATCGTGGCCAAAATCATCTGCGTTTCAGTGCCCGCAACGGGTGC
Internal bar code:CCAGTATTTGATGGCTCGATGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1354
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:13
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:GTCTGGAGTGTGCTGCTTCT
Suggested primer 2:GCACTTTCCCTTCTCGCTCT