| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.007210 |
| Chromosome: | chromosome 6 |
| Location: | 3711000 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278157 | (1 of 1) 3.2.1.143 - Poly(ADP-ribose) glycohydrolase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTTAAGTTGAGAGCAGCGAACACAGAGCGGATTTGGCGAAGTTCGCCTC |
| Internal bar code: | TCGGAGTATGAAACCCGCGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 950 |
| LEAP-Seq percent confirming: | 86.6667 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTGGAGTTAGCCCCGTTC |
| Suggested primer 2: | TGTGCCAGTGTCTACAGCTG |