| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.007220 |
| Chromosome: | chromosome 1 |
| Location: | 3453396 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g022400 | (1 of 77) IPR001623 - DnaJ domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGCATAATCGGCTACAATTAATTAAGATAACGAGCCTAGTTGTTGGTA |
| Internal bar code: | TTTAGAATGCTCGAAATATTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1021 |
| LEAP-Seq percent confirming: | 12.5 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCTCACACGCCTCTCAAC |
| Suggested primer 2: | CTCCGGTATGCTCAGACCAC |