Insertion junction: CLIP2.007222_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.007222
Chromosome:chromosome 4
Location:1412713
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g214250 AGO2 Argonaute-like protein; (1 of 3) PTHR22891:SF0 - PROTEIN ARGONAUTE-2 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCTTCGTTCTTCACATGCACTGCTATCCCCCTCATCCCCCTCCCCACTT
Internal bar code:GTAGGTTATCGCGAGGCCGTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1027
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GATACGGCAGGGTACGACAG
Suggested primer 2:ATAAACCTACCGCTCCCCCT