| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.007231 |
| Chromosome: | chromosome 3 |
| Location: | 621651 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g145507 | (1 of 1) IPR004720 - Phosphotransferase system, sorbose subfamily IIB component | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCAAAGCGAGTGTAAGATCAGTGTGCGGGTGTAGGTTGAACTGCGAGTA |
| Internal bar code: | TGTTTTGTAGGAAATTCATCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1714 |
| LEAP-Seq percent confirming: | 77.0833 |
| LEAP-Seq n confirming: | 37 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 48 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGAGAAGGCCATTGAGCT |
| Suggested primer 2: | CAGCACACGTCACAAGCAAA |