Insertion junction: CLIP2.007237_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.007237
Chromosome:chromosome 4
Location:3215350
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre04.g226450 PRP17 (1 of 1) K12816 - pre-mRNA-processing factor 17 (CDC40, PRP17); Nuclear pre-mRNA splicing factor, component of the spliceosome intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AATCATAAATGCAACCCTCCCGCGTCGCACTAAACGAACACCCCTCCAGG
Internal bar code:TCCCGTAGCTGTGTGGGGAAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:642
LEAP-Seq percent confirming:14.8148
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:23
LEAP-Seq n unique pos:27

Suggested primers for confirmation by PCR

Suggested primer 1:AAACCACGCGTCCTACCATT
Suggested primer 2:CATTCCACTGCATGATGCCG