| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.007239 |
| Chromosome: | chromosome 1 |
| Location: | 116373 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g000800 | CGL136 | Conserved in the Green Lineage | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTTCGTGGCGGCACCAGGCCGTGATGCAGGCTGACCGGCTGGCTCGGG |
| Internal bar code: | ATAGTTTTTGCTCAATGTCTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2588 |
| LEAP-Seq percent confirming: | 32.0 |
| LEAP-Seq n confirming: | 48 |
| LEAP-Seq n nonconfirming: | 102 |
| LEAP-Seq n unique pos: | 150 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTACAACTGCCGTATGCG |
| Suggested primer 2: | TGAAAAGTGCGTCTGTTGCG |