Insertion junction: CLIP2.007244_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.007244
Chromosome:chromosome 3
Location:8655324
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g209841 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGGCTACCGCGTTGCAAGGGCGCTTGGGCTGACCAGCAACCCCGCGCTC
Internal bar code:CACACAGTATGCTGTATATTCC

Confirmation - LEAP-Seq

LEAP-Seq distance:1409
LEAP-Seq percent confirming:76.9231
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:CGCACACACATGCTTGTCAA
Suggested primer 2:ATCTGATCGCTTCGCTCTGG