| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.007250 |
| Chromosome: | chromosome 6 |
| Location: | 6146289 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g291650 | FDX11 | (1 of 1) PTHR23426//PTHR23426:SF32 - FERREDOXIN/ADRENODOXIN // 2FE-2S IRON-SULFUR CLUSTER BINDING DOMAIN-CONTAINING PROTEIN; 2Fe-2S ferredoxin | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAGCTTATTATGATGAGCGACGTTAACGCCCCGCAGACGGCATGAGGAC |
| Internal bar code: | GTTACGGACAGGTCCATGAGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5805 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 80 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 80 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCATCCTCGCGATACTGCAC |
| Suggested primer 2: | CCCCTCTCAAAGCAGGTCAG |