| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.007274 |
| Chromosome: | chromosome 3 |
| Location: | 587727 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g145387 | FAP239 | Coiled-Coil Flagellar Associated Protein 239 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGTTGTGCAGCGACTCCTTCTTCTTCCGCCGCACCTGCGGGAGCAGCAG |
| Internal bar code: | CTGAACTCTGCGCAGGGCACTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3005 |
| LEAP-Seq percent confirming: | 73.913 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATGCATTGCGCGATTGTA |
| Suggested primer 2: | TAACCTGTGTAGCCTTGCGG |