| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.007286 |
| Chromosome: | chromosome 10 |
| Location: | 5628676 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g458700 | (1 of 6) PF07719//PF13414 - Tetratricopeptide repeat (TPR_2) // TPR repeat (TPR_11) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGAGGGGATGGGCTACCAGGTTTCCATTCTGCTTGCAGCGATCGTGTG |
| Internal bar code: | AGTCCTTACTTACGTACGGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4252 |
| LEAP-Seq percent confirming: | 84.4444 |
| LEAP-Seq n confirming: | 38 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 45 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCCAAACAGGCCATAGCTG |
| Suggested primer 2: | AGGCTGGCGCTTTAGAACAT |