| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.007288 |
| Chromosome: | chromosome 4 |
| Location: | 1092108 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g216100 | (1 of 1) 4.4.1.5 - Lactoylglutathione lyase / Methylglyoxalase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGTGTGATGGCAATGGGGAGACGGAAGAGGATGGGAGACCGAAAGAGAT |
| Internal bar code: | GGGGTACCTGTGGCTGAGTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 114 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTTGACACGCACACAGTT |
| Suggested primer 2: | AAATGGGAATGGGAGCTGGG |