| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.007294 |
| Chromosome: | chromosome 3 |
| Location: | 5326713 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g182900 | (1 of 1) IPR008559 - Uncharacterised conserved protein UCP023322, transmembrane eukaryotic | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCACCACCATCATCACCACGTAGCTGCGCATGCGCCACATGTAAAAGTC |
| Internal bar code: | TCAAGGAAAGAGAGCAATGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2756 |
| LEAP-Seq percent confirming: | 72.1311 |
| LEAP-Seq n confirming: | 44 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 61 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGGGAGTTCAGGAGGCTG |
| Suggested primer 2: | GGACAGCACGTACAGGAACA |