| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.007298 |
| Chromosome: | chromosome 2 |
| Location: | 3633913 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g099850 | PDC2 | (1 of 1) PTHR11516:SF25 - PYRUVATE DEHYDROGENASE E1 COMPONENT SUBUNIT ALPHA-3, CHLOROPLASTIC; Pyruvate dehydrogenase, E1 component, alpha subunit | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCGTCTGGAGGCAGGAAAGGAAGGCCAGCTGCGATGGCCATGATAGCC |
| Internal bar code: | GACGGTTCCGGGCGGTTTATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 868 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGATGGTGGGGTTGAGGTG |
| Suggested primer 2: | GGGTGGGTTGGAGGTACAAG |