| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.007306 |
| Chromosome: | chromosome 12 |
| Location: | 3452181 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g497400 | CGL119 | (1 of 1) PTHR13408:SF0 - DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC4; possible RNA polymerase III subunit | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAAGGGGGATGGTAGCACGCGGGCAGCAGCACAGCGAGGCGATTCGTCA |
| Internal bar code: | GCAAGAGCGTATACCCAATGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 180 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTTATGGGCCCAGCAGAGT |
| Suggested primer 2: | GCCCAAGCCTATCCTGTGTT |