| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.007318 |
| Chromosome: | chromosome 16 |
| Location: | 2423714 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g659850 | CGL37 | conserved protein related to shikimate kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCTTTCCTCATTGCTTGTTTCTTCTCTTCCCAATGCTACTTCCAACCTT |
| Internal bar code: | TACTATGCGTCTCTCGTCAGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1331 |
| LEAP-Seq percent confirming: | 56.7568 |
| LEAP-Seq n confirming: | 42 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 74 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCTGCTTCTACTGCTCCTG |
| Suggested primer 2: | AATGCTGACTGGGATGTGCA |