| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.007333 |
| Chromosome: | chromosome 6 |
| Location: | 3789979 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278171 | CGL132 | predicted protein; (1 of 14) PF04720 - PDDEXK-like family of unknown function (PDDEXK_6) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGTGCACAAAGTCGCCCTTCCCTGCCCGCTCGTGAAGGCCCACGCACC |
| Internal bar code: | GGGATCGGTGAATCGTCGGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2753 |
| LEAP-Seq percent confirming: | 69.3182 |
| LEAP-Seq n confirming: | 61 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 88 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACCCCATCCACGTTTTCCG |
| Suggested primer 2: | CGCGGAAGGCATCATAGACA |