| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.007346 |
| Chromosome: | chromosome 2 |
| Location: | 4396143 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g105600 | EYE3 | Eyespot assembly protein, ABC1 kinase family; (1 of 2) PTHR10566//PTHR10566:SF45 - CHAPERONE-ACTIVITY OF BC1 COMPLEX CABC1 -RELATED // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGGTTTGGACGTGAGCAGTGCAGGACGTGCCGAAGGTCGGCTAAGATC |
| Internal bar code: | ACGCGGGGGTTCCCTAGAGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1017 |
| LEAP-Seq percent confirming: | 54.8387 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTCATTGAACGCGCTCTC |
| Suggested primer 2: | TGAACGAGCACTAACAGCGT |