| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.007350 |
| Chromosome: | chromosome 3 |
| Location: | 3812239 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g169900 | (1 of 4) IPR023222 - PsbQ-like domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCCTGGACTACCAGCAGTACTTCGACAGGTGCGTGCAATTCTACTAAAA |
| Internal bar code: | TTGTATAGTTTAGCTAATCGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3583 |
| LEAP-Seq percent confirming: | 80.0 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGCCCCAGTCTACCAAAT |
| Suggested primer 2: | CATCTGGGTTGGGGCTTGAT |