Insertion junction: CLIP2.007372_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.007372
Chromosome:chromosome 8
Location:2902605
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g374300 CGL115 (1 of 1) K19323 - ataxin-10 (ATXN10); Conserved in the Green Lineage intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCCAACGCGGTACCTCTCGCCACCCCCGCTACTGCATTGCGCCCAGGTG
Internal bar code:TTGCGCGTGTCATTTTAGGTAT

Confirmation - LEAP-Seq

LEAP-Seq distance:1846
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:24
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:24

Suggested primers for confirmation by PCR

Suggested primer 1:CAAACACACCTTTCCGCCTG
Suggested primer 2:CAACCACAACAAGCGCCTAC