| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.007405 |
| Chromosome: | chromosome 14 |
| Location: | 2496005 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g624350 | VTE6 | Tocopherol O-methyltransferase; (1 of 1) K18534 - MPBQ/MSBQ methyltransferase (K18534) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGGCTTCAGATTCAGCCGCTCGCCGAGTCGCCGTCAAATTGTCGTACAC |
| Internal bar code: | TCCGCTAACTAAGATTATCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 632 |
| LEAP-Seq percent confirming: | 96.2963 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCACCGGACCCACCTCTAAT |
| Suggested primer 2: | GGAACTCCGGGGCGTTATAG |