| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.007441 |
| Chromosome: | chromosome 10 |
| Location: | 910350 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g424050 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTAAAGCTGCCGGTGGCTGCGCCCGTGGGGTGCGAAGTGTACTCGCCGG |
| Internal bar code: | TTTTTCTTTTGTATTAATCTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3039 |
| LEAP-Seq percent confirming: | 45.7143 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCAAAACCCGCATTCTTGC |
| Suggested primer 2: | CTCTGCATTGAACATCCGCG |