Insertion junction: CLIP2.007457_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.007457
Chromosome:chromosome 14
Location:3375971
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g629840 (1 of 22) PTHR11017//PTHR11017:SF145 - LEUCINE-RICH REPEAT-CONTAINING PROTEIN // SUBFAMILY NOT NAMED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCTCTGATTGGTACTGAGGCCAGGTTCTAGCTAGGCCCCCACAGCATCC
Internal bar code:AACTTGTGCCGTCGAAAAGTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1455
LEAP-Seq percent confirming:90.9091
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:CAAGCCCGCTATTGTTGACG
Suggested primer 2:TTGAACGCGCACATCGAATC