Insertion junction: CLIP2.007472_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.007472
Chromosome:chromosome 9
Location:2709840
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g391650 HCP4 (1 of 4) 1.7.99.1 - Hydroxylamine reductase / Hydroxylamine (acceptor) reductase; Hybrid-cluster protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCCAGCAAACTTTTCTCTCACCACATCATGGCGCTCTCGGCGTCCAGCA
Internal bar code:ACACGTCTTAATCATGTTATGC

Confirmation - LEAP-Seq

LEAP-Seq distance:243
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:AAGGGGCAGTGGTGATGATG
Suggested primer 2:AGGCCCTTGACCGAGTAGAT