| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.007484 |
| Chromosome: | chromosome 5 |
| Location: | 910960 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g246551 | (1 of 1) IPR000104//IPR001810//IPR020683 - Antifreeze protein, type I // F-box domain // Ankyrin repeat-containing domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGCATGCGTGCCCATACATCACATTGGAGTGGGAGTCACTTATTCACC |
| Internal bar code: | ATTGCTCTTGAAGTAATTCAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 638 |
| LEAP-Seq percent confirming: | 78.2609 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCCGGGTTAAATTGCTTGC |
| Suggested primer 2: | CACTCACACTGTCACCTCCC |