| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.007489 |
| Chromosome: | chromosome 3 |
| Location: | 5197727 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g181500 | DPE1,STA11 | Disproportionating enzyme 1; (1 of 1) PTHR32438:SF5 - 4-ALPHA-GLUCANOTRANSFERASE DPE1, CHLOROPLASTIC/AMYLOPLASTIC | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGTAACACGGCTGCTCAATCACGCTGCTCCATCTTGTGTCCCGCATTGC |
| Internal bar code: | GCACCGTGGCGAAAGAAACGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 242 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATAGGGACTCGTGCTGCAC |
| Suggested primer 2: | CCTTGTACCGTTCCGTGTCA |