| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.007504 |
| Chromosome: | chromosome 13 |
| Location: | 1959266 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g576350 | PFP2 | Prefoldin-family protein; (1 of 1) K17560 - unconventional prefoldin RPB5 interactor 1 (URI1) | 3'UTR |
| Cre13.g576400 | ADCL1,BCA4 | aminotransferase related to 4-amino-4-deoxychorismate lyase; (1 of 1) 2.6.1.21//4.1.3.38 - D-amino-acid transaminase / D-aspartic aminotransferase // Aminodeoxychorismate lyase / Enzyme X | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAAGGCTGCATTGGCATGGGGCGCATGGGCCGGGTGGTGCATGTAAACC |
| Internal bar code: | GCCTTGCTACAAGGGAGGTCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5839 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 92 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 92 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCATGTGGCTGCTGTAGAA |
| Suggested primer 2: | TCGAGGAAAGCCACTGTGAC |