| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.007505 |
| Chromosome: | chromosome 16 |
| Location: | 4246212 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g669650 | (1 of 2) 3.5.99.10 - 2-iminobutanoate/2-iminopropanoate deaminase / Enamine/imine deaminase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACAAGGATGTGGTGAAGACCACCATCCTGCTGGTGAGCTCAGAGCACGG |
| Internal bar code: | ACCTAAGGTGCCGAGTTGGACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1857 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAGAAGGGAGGGAGGGATG |
| Suggested primer 2: | CAGCTTTGTTCGTTGCCCTC |