| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.007524 |
| Chromosome: | chromosome 6 |
| Location: | 6890097 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g296600 | RFS1 | Riboflavin synthase; (1 of 1) 2.5.1.9 - Riboflavin synthase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGGTTGAAGGGCGGCCCTCCACTCACGGCTCACAATCTTGAGATTGGC |
| Internal bar code: | TATCGGGGGTTGCATACAGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1496 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAGGAGTTGGAGGCCACAC |
| Suggested primer 2: | CGGCATGACGCATACTGTTG |