| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.007525 |
| Chromosome: | chromosome 6 |
| Location: | 7407159 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g300250 | TTL10 | (1 of 1) K16600 - tubulin polyglutamylase TTLL2 [EC:6.-.-.-] (TTLL2); Putative tubulin tyrosine ligase | outside_mRNA |
| Cre06.g300300 | (1 of 39) IPR009003 - Peptidase S1, PA clan | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTAAGCTCCCTTCTGTAATGAACCCCCGAGGCGGCGGAGGACAAGTGT |
| Internal bar code: | CATTAGGGATGTAGTTACGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1838 |
| LEAP-Seq percent confirming: | 88.5714 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTCACTCTACCCTCCCGAC |
| Suggested primer 2: | CGTGGAAAACAGCGGACTTG |