Insertion junction: CLIP2.007544_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.007544
Chromosome:chromosome 2
Location:5005797
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g110750 LHCB7,LHCQ Light-harvesting chlorophyll a-b binding protein, chloroplast precursor; (1 of 1) K14172 - light-harvesting complex II chlorophyll a/b binding protein 7 (LHCB7) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGACTCTCCTTACGCAGTGAATCAGGAAAGGTAGGGGCATCTGTTGAGC
Internal bar code:TAAATCTGTAGCTGCTCTCCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:2866
LEAP-Seq percent confirming:57.1429
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:ATCACATTTATGCGCACGCC
Suggested primer 2:GTCCCTAGGCTCCAGTGGTA