Insertion junction: CLIP2.007549_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.007549
Chromosome:chromosome 17
Location:3539864
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g724700 PAO3,Tic55-like3,TIC55-3,PAO1 Pheophorbide a oxygenase-related protein; (1 of 3) K13071 - pheophorbide a oxygenase (PAO, ACD1) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCGAATTCACACGGCTCCACCGGTGCCCGTTGGTTGCATGGTTGACAAG
Internal bar code:CGTTGTTTCACGATTTTTGCCC

Confirmation - LEAP-Seq

LEAP-Seq distance:3302
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:84
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:84

Suggested primers for confirmation by PCR

Suggested primer 1:GAACATTGTCGGATGGGGGT
Suggested primer 2:GCGCATCTGCTTACGTTGAG