Insertion junction: CLIP2.007559_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.007559
Chromosome:chromosome 12
Location:4310492
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCGACCGAACTTCCCGCCGTTGACGGTTTCCCCGCACTCGCTCGCCAAT
Internal bar code:CTTATCCGTTAGGACTTGAGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:3859
LEAP-Seq percent confirming:93.9394
LEAP-Seq n confirming:62
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:66

Suggested primers for confirmation by PCR

Suggested primer 1:CGACTCCTCTCGATGAGCAC
Suggested primer 2:CGTCGTCTTTCCCACACTCA