Insertion junction: CLIP2.007570_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.007570
Chromosome:chromosome 16
Location:5891617
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre16.g679300 CGL139 Conserved in the Green Lineage 3'UTR
Cre16.g679350 (1 of 3) IPR003131//IPR011333 - Potassium channel tetramerisation-type BTB domain // POZ domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCAATGGGTCGGTGACTCCGTGTACGTCACTGCCTCACATCCTCCCTACG
Internal bar code:CTTGCTGTCCAACAGAACGGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:730
LEAP-Seq percent confirming:55.5556
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:CACCTACCGTATCACTGCCC
Suggested primer 2:CTGGGCGCCATTCTAAAGGA