| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.007589 |
| Chromosome: | chromosome 12 |
| Location: | 9271055 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g542569 | (1 of 4) PF00497 - Bacterial extracellular solute-binding proteins, family 3 (SBP_bac_3) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCGGAAGTGCTTGCGTGGTCATCGGGTGGGGCGTGAGGCGTGTGTCAGC |
| Internal bar code: | GTATTGTTCTGCTCCATTGACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2469 |
| LEAP-Seq percent confirming: | 79.5455 |
| LEAP-Seq n confirming: | 35 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGCTCATCCTCCTCCTCTT |
| Suggested primer 2: | CGCTACCCCACACGTATCTC |