Insertion junction: CLIP2.007590_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.007590
Chromosome:chromosome 17
Location:4486873
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g731100 CPL14 (1 of 8) PF10184 - Uncharacterized conserved protein (DUF2358) (DUF2358); Conserved in the Plant Lineage CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACACGGCTCATATCGTGAATCGCTGTGGCGCACGGCCTCGCCACCCGCAT
Internal bar code:TGATGCCTTATCGATTCCCGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:388
LEAP-Seq percent confirming:9.09091
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:GGCATTTGCAGCAACAGTGA
Suggested primer 2:ATTGCTTAACGCTCGGGTCA