| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.007610 |
| Chromosome: | chromosome 13 |
| Location: | 4421930 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g602350 | (1 of 1) K01609 - indole-3-glycerol phosphate synthase (trpC) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATGTTCTGTCATAATTCTCAAATCACCCTCTTATAATAAGCCTGTGGCT |
| Internal bar code: | TGGCGCAGTGATTGTGTATTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1654 |
| LEAP-Seq percent confirming: | 50.0 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAACATGATGCACGTCGAC |
| Suggested primer 2: | GCAGGGCCTAGCAAAGAAGA |